Extracts of Peltophorum africanum had good antioxidant activity with IC values of 4.67 ± 0.31 μg/m L and 7.71 ± 0.36 μg/m L compared to that of the positive control ascorbic acid (2.92 ± 0.14 μg/m L and 13.57 ± 0.44 μg/m L), using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) methods, respectively.
The metabolism of linoleic acid to leukotriene derivatives by 15-lipoxygenase (15-LOX) was also inhibited by the crude acetone extracts of Peltophorum africanum (IC = 0.05), indicating that flavonoids are not involved in LOX inhibition.
Inflammation is a common risk factor in the pathogenesis of conditions such as infections, arthritis, type 2 diabetes mellitus, obesity and cancer.
An ethnobotanical survey of medicinal plants used traditionally to treat inflammation and related disorders such as pain, arthritis and stomach aches in southern Africa led to the selection of 25 plant species used in this study.
Thus, therapeutic effects of SDG in microglia may be attributed to other mechanisms.
Changes in protocol may prove helpful in finding an interaction but it is recommended that future investigation of SDG’s effect on microglia focus on other pathways.
ELISA, nitrate-nitrite assay, and q PCR analyses probing for IL-1β and IL-6, nitrate and nitrite, and TNF-α and IL-1β, respectively, were performed to measure NF-κB activity.
The q PCR results show a significant increase in m RNA expression of IL-1β and TNF-α after treatment with 10 ng/m L TNF-α ().
Evidence-based research supports the medical and pharmacological benefits of plant-derived compounds, with increasing interest in the identification and characterization of bioactive compounds from natural sources .
One of the earliest recorded approaches for treating inflammation and pain was the application of extracts from willow leaves by Celsius in 30 AD .